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Remarkable Bactericidal Efficacy of Plumeria Rubra Extract Mediated ZnO Nanoparticles

Students & Supervisors

Student Authors
Syeda Tanha Hossain Progga
Bachelor of Science in Computer Science & Engineering, FST
Supervisors
Dr. Farzana Sabeth
Associate Professor, Faculty, FST
Ms. Bithi Paul
Assistant Professor, Faculty, FST

Abstract

The phytosynthesis of zinc oxide (ZnO) nanoparticles (NPs) has gained significant attention in advanced research due to their unique physicochemical properties and versatile applications in drug delivery, cosmetics, biosensors, and antibacterial formulations. With the increasing challenge of microbial contamination and the emergence of multidrug-resistant pathogens recognized as serious global health threats, developing effective antimicrobial agents is of paramount importance. In this study, a simple, single-pot green synthesis of ZnO nanoparticles (KG-ZnO) using the aqueous flower extract of Plumeria rubra (commonly known as Kathgolap) as a natural reducing, capping, and stabilizing agent is presented, along with an assessment of their antibacterial activity against four bacterial strains. The aqueous flower extract facilitated an eco-friendly synthesis of KG-ZnO MNPs. The structural, optical, and morphological characteristics of the synthesized nanoparticles were analyzed using UV–Visible spectroscopy (UV-Vis), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM) with energy dispersive X-ray spectroscopy (EDX), and transmission electron microscopy (TEM). Antibacterial efficacy was evaluated via the disc diffusion method on Mueller-Hinton agar, with inhibition zones measured after 24 hours at 37°C. XRD with Rietveld refinement confirmed a hexagonal wurtzite structure with crystallite sizes of 51 nm, while UV–Vis analysis indicated a band gap of 2.98 eV. The KG-ZnO nanoparticles demonstrated notable antibacterial activity against both Gram-positive and Gram-negative bacteria. Specifically, inhibition zones for E. coli were 11.5 mm and 12.5 mm, P. aeruginosa 8.5 mm and 10 mm, S. flexneri 9.0 mm and 10.5 mm, and S. aureus 9.0 mm and 10.0 mm at 200 μg/disk and 400 μg/disk, respectively. These results highlight the potential of green-synthesized KG-ZnO nanoparticles as effective bactericidal agents.

Keywords

Flower extract nanoparticles antibacterial activity Inhibition Zone

Publication Details

  • Type of Publication:
  • Conference Name: 1st International Conference on Life and Earth Sciences for Sustainable Development (ICLESSD-2025)
  • Date of Conference: 18/12/2025 - 18/12/2025
  • Venue: Jagannath University
  • Organizer: Faculty of Life and Earth Sciences, Jagannath University
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